核酸扩增仪中温度的控制研究

Temperature Control Method in Application of Nucleic Acid Amplification

中文关键词:  核酸扩增仪  温度控制  PID  自抗扰算法

英文关键词:nucleic acid amplification  temperature control  PID  ADRC

中文摘要:

      核酸扩增仪一直是各种生命科学研究中重要的基础仪器设备之一。在核酸扩增仪中温度的快速性、精确性是影响其扩增效果的重要因素。选取在核酸扩增仪中的96孔反应槽作为研究平台，采用半导体制冷制热片对系统进行加热和降温。目前，已有大量应用实践证明自抗扰控制器的控制精度、抗干扰能力、参数鲁棒性等方面性能优于PID控制器，通过LabVIEW进行自抗扰控制和传统PID控制算法编程，对核酸扩增仪中实验平台进行快速升降温实验，并以实验数据来判断两种控制在核酸扩增仪中控制效果的优劣。

英文摘要:

      Nucleic acid amplification has been one of a variety of important basic equipments for life science research.Rapid temperature response and accuracy are important factors that affect the amplification effect.A 96-well reaction block is chosen as the study platform，and the semiconductor chilling plate is used to heat and cool the system.Currently,there are a large nunber of applications which prove that ADRC controller outperforms the traditional PID controller.The pros and cons between ADRC and PID controllers for the block thermal control in the application of the nucleic acid amplification are presented based on the experiment data acquired with algorithms programmed in LabVIEW.